Atomic mutagenesis reveals A2660 of 23S ribosomal RNA as key to EF-G GTPase activation.

@article{Clementi2010AtomicMR,
  title={Atomic mutagenesis reveals A2660 of 23S ribosomal RNA as key to EF-G GTPase activation.},
  author={Nina Clementi and Anna Chirkova and Barbara Puffer and Ronald Micura and Norbert Polacek},
  journal={Nature chemical biology},
  year={2010},
  volume={6 5},
  pages={344-51}
}
Following ribosomal peptide bond formation, the reaction products, peptidyl-tRNA and deacylated tRNA, need to be translocated from the A- and P-sites to the P- and E-sites, respectively. This process is facilitated by the GTPase elongation factor G (EF-G). The mechanism describing how the ribosome activates GTP hydrolysis is poorly understood in molecular terms. By using an 'atomic mutagenesis' approach, which allows the manipulation of specific functional groups on 23S rRNA nucleotides in the… CONTINUE READING