Atomic mutagenesis of stop codon nucleotides reveals the chemical prerequisites for release factor-mediated peptide release

@inproceedings{Hoernes2018AtomicMO,
  title={Atomic mutagenesis of stop codon nucleotides reveals the chemical prerequisites for release factor-mediated peptide release},
  author={Thomas Philipp Hoernes and Nina Clementi and Michael Andreas Juen and Xinying Shi and Klaus Faserl and Jessica Willi and Catherina Gasser and Christoph Kreutz and Simpson Joseph and Herbert Lindner and Alexander H{\"u}ttenhofer and Matthias David Erlacher},
  booktitle={Proceedings of the National Academy of Sciences of the United States of America},
  year={2018}
}
Termination of protein synthesis is triggered by the recognition of a stop codon at the ribosomal A site and is mediated by class I release factors (RFs). Whereas in bacteria, RF1 and RF2 promote termination at UAA/UAG and UAA/UGA stop codons, respectively, eukaryotes only depend on one RF (eRF1) to initiate peptide release at all three stop codons. Based on several structural as well as biochemical studies, interactions between mRNA, tRNA, and rRNA have been proposed to be required for stop… CONTINUE READING