Atomic force microscopy to study direct neurite-mast cell (RBL) communication in vitro.

@article{Ohshiro2000AtomicFM,
  title={Atomic force microscopy to study direct neurite-mast cell (RBL) communication in vitro.},
  author={H Ohshiro and Ryota Suzuki and Tadahide Furuno and Mamoru Nakanishi},
  journal={Immunology letters},
  year={2000},
  volume={74 3},
  pages={211-4}
}
Communication between nerves and mast cells is a prototypic demonstration of neuroimmune interaction. We used an in vitro co-culture approach comprising cultured murine superior cervical ganglia (SCG) and rat basophilic leukemia (RBL-2H3) cells. Atomic force microscopy (AFM) showed how neurites attached to a pseudopodium or a cell body of an RBL cell. After stimulation of SCG neurites with bradykinin or scorpion venom, RBL cells attached to neurites spread and flattened, and several discharged… CONTINUE READING

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