Atomic force microscopy analysis of icosahedral virus RNA.

@article{Kuznetsov2005AtomicFM,
  title={Atomic force microscopy analysis of icosahedral virus RNA.},
  author={Yurii G. Kuznetsov and Sarah Daijogo and Jiashu Zhou and Bert L Semler and Anastasia Mcpherson},
  journal={Journal of molecular biology},
  year={2005},
  volume={347 1},
  pages={
          41-52
        }
}
Single-stranded genomic RNAs from four icosahedral viruses (poliovirus, turnip yellow mosaic virus (TYMV), brome mosaic virus (BMV), and satellite tobacco mosaic virus (STMV)) along with the RNA from the helical tobacco mosaic virus (TMV) were extracted using phenol/chloroform. The RNAs were imaged using atomic force microscopy (AFM) under dynamic conditions in which the RNA was observed to unfold. RNAs from the four icosahedral viruses initially exhibited highly condensed, uniform spherical… CONTINUE READING
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