Atomic force microscopy analysis of cell volume regulation.

@article{Spagnoli2008AtomicFM,
  title={Atomic force microscopy analysis of cell volume regulation.},
  author={Chiara Spagnoli and Arthur Beyder and Stephen R. Besch and Frederick Sachs},
  journal={Physical review. E, Statistical, nonlinear, and soft matter physics},
  year={2008},
  volume={78 3 Pt 1},
  pages={031916}
}
Cells swell in response a hypoosmotic challenge. By converting osmotic pressure to hydrostatic pressure at the cell membrane via van't Hoff's law, and converting that to tension via Laplace's law one predicts that the cell membrane should stretch and become stiff. We tested this prediction using the atomic force microscopy. During osmotic swelling cells did not become stiff and generally became softer. This result contradicts the assumption of the cell membrane as the constraining element in… CONTINUE READING

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