Atomic force microscopy: a novel approach to the detection of nanosized blood microparticles

  title={Atomic force microscopy: a novel approach to the detection of nanosized blood microparticles},
  author={Yuana Yuana and Tjerk H Oosterkamp and Svetlana Bahatyrova and Brian Ashcroft and P Garcia Rodriguez and Rogier M. Bertina and Susanne Osanto},
  journal={Journal of Thrombosis and Haemostasis},
See also Freyssinet J‐M, Toti F. Membrane microparticle determination: at least seeing what’s being sized! This issue, pp 311–4. 

Membrane microparticle determination: at least seeing what’s being sized!

Atomic force microscopy: a novel approach to the detection of nanosized blood microparticles and its applications in medicine and materials science.

Single vs. swarm detection of microparticles and exosomes by flow cytometry

See also Harrison P, Gardiner C. Invisible vesicles swarm within the iceberg. This issue, pp 916‐8.

Particle size distribution of exosomes and microvesicles determined by transmission electron microscopy, flow cytometry, nanoparticle tracking analysis, and resistive pulse sensing

Enumeration of extracellular vesicles has clinical potential as a biomarker for disease and currently employed techniques detect concentrations ranging from 104 to 1012 vesicle mL–1.

Exosomes and Microvesicles : An introduction Nanoparticle Tracking Analysis ( NTA ) Overview

  • Physics
  • 2014
NTA utilizes the properties of both light scattering and Brownian motion in order to obtain the particle size distribution of samples in liquid suspension. A laser beam is passed through the sample

Methods to Analyze EVs.

This chapter aims to summarize physical principles of novel and conventional technologies to be used in the EV field and to discuss advantages and limitations.

Impedance-based flow cytometry for the measurement of microparticles.

  • J. Zwicker
  • Biology
    Seminars in thrombosis and hemostasis
  • 2010
This review details the rationale for employing impedance-based flow cytometry in the measurement of blood-borne microparticles and offers a sensitive methodology to characterizemicroparticles.

Circulating microparticles: challenges and perspectives of flow cytometric assessment.

Circulating blood microparticles are likely to play a significant role as messengers of biological information. Their accurate quantification and characterisation is challenging and needs to be

The detection of tissue factor bearing-microparticles by atomic force microscopy

TF-bearing MPs could be captured on mica coated with anti-TF antibody and sensitively be detected by AFM operated in fluid tapping mode and it is proposed that this method may be used for the detection of TF-positive MPs in patient samples.



Membrane microparticles: two sides of the coin.

Microparticles are plasma membrane-derived vesicles shed from stimulated cells, in the broad sense of the term. Their presence is interpreted by proximal or remote cells in fundamental physiological

Adsorption of biological molecules to a solid support for scanning probe microscopy.

The commonly used method of adsorbing biological specimens to freshly cleaved mica is analysed, facilitated by adjusting the electrolyte concentration and the pH of the buffer solution.

From molecules to cells: imaging soft samples with the atomic force microscope.

An overview is presented on the application of atomic force microscopy to organic samples ranging from thin ordered films at molecular resolution to living cells and novel imaging modes are introduced that exploit different aspects of the tip-sample interaction for local measurements of the micromechanical properties of the sample.

Detection and measurement of microparticles: an evolving research tool for vascular biology.

It now appears that all circulating blood cells as well as endothelial cells release membranous fragments ~1 mum in size or smaller bearing at least some characteristics of the parent cell.

Past, present and future of atomic force microscopy in life sciences and medicine

Recent progress made using AFM for studying cells and cellular interactions, probing single molecules, imaging biosurfaces at high resolution and investigating model membranes and their interactions are presented.

Standardization of platelet‐derived microparticle counting using calibrated beads and a Cytomics FC500 routine flow cytometer: a first step towards multicenter studies?

This calibrated‐bead strategy allowed full long‐term control of the FCM‐based microparticle protocol and reproducible PMP counts over time and opens the way for multicenter studies.

Tissue factor-bearing microparticles and cancer.

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  • Biology, Engineering
    Seminars in thrombosis and hemostasis
  • 2008
This work is using an impedance-based flow cytometer to accurately size and enumerate TF-bearing microparticles to explore the association between cancer thrombosis and elevations in circulating TF- Bearing Microparticles.

Cell-derived microparticles in haemostasis and vascular medicine.

The literature regarding microparticles in their widest definition, including ectosomes and exosomes, are reviewed, to focus mainly on their role in haemostasis and vascular medicine.