Atomic force microscopic analysis of the binding of the Schizosaccharomyces pombe origin recognition complex and the spOrc4 protein with origin DNA.

@article{Gaczynska2004AtomicFM,
  title={Atomic force microscopic analysis of the binding of the Schizosaccharomyces pombe origin recognition complex and the spOrc4 protein with origin DNA.},
  author={Maria E. Gaczynska and Pawel A. Osmulski and Ying Jiang and J K Lee and Vladimir P. Bermudez and Jerard Hurwitz},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2004},
  volume={101 52},
  pages={17952-7}
}
In eukaryotes, the initiation of DNA replication requires the interaction between origin sequences and the origin recognition complex (ORC), which is highly conserved. In this report, atomic force microscopy (AFM) was used to examine the binding of Schizosaccharomyces pombe (sp) ORC and the spOrc4 protein with the sp autonomously replicating sequence 1 (ars1). AFM imaging revealed that spORC binding to ars1 occurred solely through spOrc4p and depended on the N-terminal AT-hook domains present… CONTINUE READING
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