Atomic Positions in Rhombohedral 2-Zinc Insulin Crystals

@article{Blundell1971AtomicPI,
  title={Atomic Positions in Rhombohedral 2-Zinc Insulin Crystals},
  author={T. Blundell and J. Cutfield and S. Cutfield and E. Dodson and G. Dodson and D. Hodgkin and D. Mercola and M. Vijayan},
  journal={Nature},
  year={1971},
  volume={231},
  pages={506-511}
}
Atomic positions in three dimensions have been derived for the two non-identical molecules of insulin in rhombohedral 2-zinc insulin crystals. Interesting correlations appear in their relation to the sequence variations observed so far in natural insulins. 

Topics from this paper

Rhombohedral insulin crystal transformation.
Abstract By increasing the chloride concentration in the surrounding liquid to greater than 1 m it is possible to convert rhombohedral 2 Zn insulin crystals to 4 Zn insulin crystals; removingExpand
A preliminary Raman spectroscopic study of native zinc-insulin crystals.
TLDR
A Raman spectrum of rhombohedral zinc-insulin crystals is reported and partially interpreted, finding that the intrachain disulfide bond, A6-11, may have a local geometry different from that of the interchain disulfides, B7-A7 and B19-A20. Expand
THE CONFORMATIONS OBSERVED IN THE N TERMINAL A CHAIN RESIDUES OF INSULIN
Abstract The two independent molecules in the asymmetric unit of the 2 zinc insulin crystal assume distinctly different conformations at the A chain N terminus. The variation seems to arise from theExpand
Simulation of conformational changes in 2 Zn insulin.
TLDR
The results suggest that insulin would tend to adopt a molecule 1-like conformation in the absence of crystal packing forces, and that dimer formation is not at the origin of the observed asymmetry in the 2 Zn crystal. Expand
Helix movements in proteins
Abstract Close-packed α-helices in proteins can move relative to each other by up to ∼1.5 A by small conformational adjustments in the side-chains that form the interface between them. Such relativeExpand
Low resolution crystal structure of hagfish insulin.
TLDR
The application of the Rossmann & Blow (1962) rotation function has established the existence of an insulin dimer containing a crystallographic 2-fold axis from the Atlantic hagfish, Myxine glutinosa, crystallizes in space group P41212 with a monomer in the asymmetric unit. Expand
Water of co-ordination in insulin.
TLDR
This calculation of interatomic separation shows that it is essential to take into account the effect on the radial factor of the unpaired cupric spin delocalization onto the water oxygen, which was quantitated from the ligand hyperfine splitting arising from the co-ordinated nitrogens. Expand
Structure of insulin in 4-zinc insulin
TLDR
It is found, from electron density calculations on 4-zinc pig insulin, that the apparent similarity of the crystal structures conceals a surprisingly large change in the conformation of one only of the two insulin molecules in the unit. Expand
Receptor-binding region of insulin
X-ray analysis, circular dichroism, receptor binding and biological potencies of chemically modified insulins suggest that the conformation of the insulin molecule is critical to the formation ofExpand
Transmission of conformational change in insulin
TLDR
Crystal structures of insulin contain molecules that are similar but not identical in conformation, which limits the extent to which conformational changes can be dissipated locally, causing their transmission over long distances. Expand
...
1
2
3
4
5
...

References

SHOWING 1-7 OF 7 REFERENCES
Structure of Rhombohedral 2 Zinc Insulin Crystals
An electron density map has been calculated at a resolution of 2.8 Å which shows many details of the arrangement of the atoms in rhombohedral crystals of insulin.
The binding of transition metal ions in insulin crystals.
TLDR
X-ray diffraction data are presented which suggest that cupric insulin crystals are isomorphous with the much studied zinc ones, and the two-nitrogen hyperfine pattern which is observed is seen to be a consequence of metal coordination to three nitrogens. Expand
A mathematical model-building procedure for proteins
to be 0.112 A shorter than the sum of the Pauling covalent radii for carbon and sulphur. This bond must therefore possess considerable double-bond character. Cucka (1963) also puts forward anExpand
Species variation in the amino acid sequence of insulin.
  • L. Smith
  • Medicine
  • The American journal of medicine
  • 1966
TLDR
In the course of analyzing rat insulin isolated from a large number of rat pancreases it was found that three components were obtained after oxidation with performic acid and electrophoresis at pH 9 in 1 per cent ammonium. Expand
The matching of physical models to three-dimensional electron-density maps: a simple optical device.
  • F. Richards
  • Physics, Medicine
  • Journal of molecular biology
  • 1968
Abstract A simple device is described which permits the optical superposition of skeleton atomic models and three-dimensional electron-density maps. The model of a globular protein can be builtExpand