Atlantic halibut (Hippoglossus hippoglossus) vitellogenin: induction, isolation and partial characterization

  title={Atlantic halibut (Hippoglossus hippoglossus) vitellogenin: induction, isolation and partial characterization},
  author={Birgitta Norberg},
  journal={Fish Physiology and Biochemistry},
Vitellogenin (VTG) synthesis was induced by repeated injections of estradiol-17β in juvenile Atlantic halibut (Hippoglossus hippoglossus). VTG eluted as a large, phosphoprotein containing peak on DEAE-Sephacel chromatography of plasma from estradiol-17β treated juvenile and mature female, but not mature male halibut. A purification procedure for Atlantic halibut VTG was developed, where VTG was precipitated with MgCl2, EDTA and distilled water, and the precipitated protein submitted to anion… CONTINUE READING
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