AtRTD – a comprehensive reference transcript dataset resource for accurate quantification of transcript‐specific expression in Arabidopsis thaliana

@inproceedings{Zhang2015AtRTDA,
  title={AtRTD – a comprehensive reference transcript dataset resource for accurate quantification of transcript‐specific expression in Arabidopsis thaliana
},
  author={Runxuan Zhang and Cristiane P. G. Calixto and Nikoleta A Tzioutziou and Allan B James and Craig G Simpson and Wenbin Guo and Yamile Marquez and Maria Kalyna and Rob Patro and Eduardo Eyras and Andrea Barta and Hugh G Nimmo and John W. S. Brown},
  booktitle={The New phytologist},
  year={2015}
}
RNA-sequencing (RNA-seq) allows global gene expression analysis at the individual transcript level. Accurate quantification of transcript variants generated by alternative splicing (AS) remains a challenge. We have developed a comprehensive, nonredundant Arabidopsis reference transcript dataset (AtRTD) containing over 74 000 transcripts for use with algorithms to quantify AS transcript isoforms in RNA-seq. The AtRTD was formed by merging transcripts from TAIR10 and novel transcripts identified… CONTINUE READING