Asymmetrizing an icosahedral virus capsid by hierarchical assembly of subunits with designed asymmetry

  title={Asymmetrizing an icosahedral virus capsid by hierarchical assembly of subunits with designed asymmetry},
  author={Zhongchao Zhao and Joseph C.-Y. Wang and Mi Zhang and Nicholas A. Lyktey and Martin F. Jarrold and Stephen C. Jacobson and Adam Zlotnick},
  journal={Nature Communications},
Symmetrical protein complexes are ubiquitous in biology. Many have been re-engineered for chemical and medical applications. Viral capsids and their assembly are frequent platforms for these investigations. A means to create asymmetric capsids may expand applications. Here, starting with homodimeric Hepatitis B Virus capsid protein, we develop a heterodimer, design a hierarchical assembly pathway, and produce asymmetric capsids. In the heterodimer, the two halves have different growth… 

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