Asymmetric synthesis of chiral amines with omega-transaminase.

  title={Asymmetric synthesis of chiral amines with omega-transaminase.},
  author={Jun-Seop Shin and Byung Gon Kim},
  journal={Biotechnology and bioengineering},
  volume={65 2},
The asymmetric synthesis of chiral amines using prochiral ketones was carried out with (S)-specific omega-transaminase (omega-TA) from Vibrio fluvialis JS17. This reaction is inhibited severely by both products, (S)-amine and deaminated ketone. In addition, thermodynamic equilibrium strongly favored the reverse reaction. L-Alanine proved to be the best amino donor based on easy removal of the products. Optimal pH of the reactions with both whole cells and cell-free extract was 7. Amino acceptor… CONTINUE READING
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