Asymmetric somatic hybrid plants between Medicago sativa L. (alfalfa, lucerne) and Onobrychis viciifolia Scop. (sainfoin)

@article{Li1993AsymmetricSH,
  title={Asymmetric somatic hybrid plants between Medicago sativa L. (alfalfa, lucerne) and Onobrychis viciifolia Scop. (sainfoin)},
  author={Y. -G. Li and Gregory J. Tanner and A. C. Delves and Philip J. Larkin},
  journal={Theoretical and Applied Genetics},
  year={1993},
  volume={87},
  pages={455-463}
}
This paper reports on the production of intergeneric somatic hybrid plants between two sexually incompatible legume species. Medicago sativa (alfalfa, lucerne) leaf protoplasts were inactivated by lethal doses of iodoacetamide. Onobrychis viciifolia (sainfoin) suspension-cell protoplasts were gamma-irradiated at lethal doses. Following electrofusion under optimized conditions about 50,000 viable heterokaryons were produced in each test. The fusion products were cultured with the help of alfalfa… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 11 extracted citations

Plant regeneration and genetic transformation of Lotus angustissimus

Plant Cell, Tissue and Organ Culture • 2004
View 1 Excerpt