Asymmetric parental genome engineering by Cas9 during mouse meiotic exit

@inproceedings{Suzuki2014AsymmetricPG,
  title={Asymmetric parental genome engineering by Cas9 during mouse meiotic exit},
  author={Toru Suzuki and Maki Asami and Anthony C. F. Perry},
  booktitle={Scientific reports},
  year={2014}
}
Mammalian genomes can be edited by injecting pronuclear embryos with Cas9 cRNA and guide RNA (gRNA) but it is unknown whether editing can also occur during the onset of embryonic development, prior to pronuclear embryogenesis. We here report Cas9-mediated editing during sperm-induced meiotic exit and the initiation of development. Injection of unfertilized, mouse metaphase II (mII) oocytes with Cas9 cRNA, gRNA and sperm enabled efficient editing of transgenic and native alleles. Pre-loading… CONTINUE READING
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