Asymmetric division of Hansenula polymorpha reflected by a drop of light scatter intensity measured in batch microtiter plate cultivations at phosphate limitation

  title={Asymmetric division of Hansenula polymorpha reflected by a drop of light scatter intensity measured in batch microtiter plate cultivations at phosphate limitation},
  author={Kirsten Kottmeier and Jost Weber and Carsten M{\"u}ller and Thomas Bley and Jochen B{\"u}chs},
  journal={Biotechnology and Bioengineering},
Hansenula polymorpha RB11 pC10‐FMD (PFMD− GFP) (FMD promoter gfp gene) was simultaneously cultivated in the Respiration Activity Monitoring System (RAMOS) and in the microtiter plate cultivation system “BioLector” under phosphate limitation. The light scatter signal of the BioLector, for the determination of the biomass concentration in the wells, shows a significant decrease with the onset of the phosphate limitation until a stationary level is reached. At lower initial phosphate concentration… 

Prediction of Escherichia coli expression performance in microtiter plates by analyzing only the temporal development of scattered light during culture

This is the first work presenting a method for the general prediction of expression performance of E. coli based solely on the temporal development of scattered light signals and predictions of the standardized expression performance are possible.

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Flow Cytometry and Phytochemical Analysis of a Sunflower Cell Suspension Culture in a 5-L Bioreactor

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Cell adsorption control by culture conditions

The inducible variation of the surface properties of Corynebacterium glutamicum can be exploited to control a continuous reactor with adsorbed cells, resulting in a variation of flocculation and adsorption behaviour of the bacteria.

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These results are discussed in relation to the reported functions of potassium in the growth of micro-organisms, and the organizational differences between prokaryotic and eukaryotic cells.

A flow injection flow cytometry system for on-line monitoring of bioreactors.

For direct and on-line study of the physiological states of cell cultures, a robust flow injection system has been designed and interfaced with flow cytometry (FI-FCM). The core of the flow injection

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Growth dynamics of mammalian cells monitored with automated cell cycle staining and flow cytometry

  • Greg SittonF. Srienc
  • Biology
    Cytometry. Part A : the journal of the International Society for Analytical Cytology
  • 2008
A single step 15‐min DNA staining protocol is implemented using automated flow cytometry to accurately observe high‐frequency events during transient cell cycle kinetics and should be useful in studying cell cycle perturbations in response to different environmental conditions resulting from exposure to specific nutrients or to drugs.