Asymmetric division of Hansenula polymorpha reflected by a drop of light scatter intensity measured in batch microtiter plate cultivations at phosphate limitation

@article{Kottmeier2009AsymmetricDO,
  title={Asymmetric division of Hansenula polymorpha reflected by a drop of light scatter intensity measured in batch microtiter plate cultivations at phosphate limitation},
  author={Kirsten Kottmeier and Jost Weber and Carsten M{\"u}ller and Thomas Bley and Jochen B{\"u}chs},
  journal={Biotechnology and Bioengineering},
  year={2009},
  volume={104}
}
Hansenula polymorpha RB11 pC10‐FMD (PFMD− GFP) (FMD promoter gfp gene) was simultaneously cultivated in the Respiration Activity Monitoring System (RAMOS) and in the microtiter plate cultivation system “BioLector” under phosphate limitation. The light scatter signal of the BioLector, for the determination of the biomass concentration in the wells, shows a significant decrease with the onset of the phosphate limitation until a stationary level is reached. At lower initial phosphate concentration… 

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