Asymmetric Unwrapping of Nucleosomes under Tension Directed by DNA Local Flexibility

@article{Ngo2015AsymmetricUO,
  title={Asymmetric Unwrapping of Nucleosomes under Tension Directed by DNA Local Flexibility},
  author={Thuy T.M. Ngo and Qiucen Zhang and Ruobo Zhou and Jaya G. Yodh and Taekjip Ha},
  journal={Cell},
  year={2015},
  volume={160},
  pages={1135-1144}
}
Dynamics of the nucleosome and exposure of nucleosomal DNA play key roles in many nuclear processes, but local dynamics of the nucleosome and its modulation by DNA sequence are poorly understood. Using single-molecule assays, we observed that the nucleosome can unwrap asymmetrically and directionally under force. The relative DNA flexibility of the inner quarters of nucleosomal DNA controls the unwrapping direction such that the nucleosome unwraps from the stiffer side. If the DNA flexibility… CONTINUE READING

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