Association of yeast transporters with detergent-resistant membranes correlates with their cell-surface location.

@article{Lauwers2006AssociationOY,
  title={Association of yeast transporters with detergent-resistant membranes correlates with their cell-surface location.},
  author={Elsa Lauwers and Bruno Andr{\'e}},
  journal={Traffic},
  year={2006},
  volume={7 8},
  pages={1045-59}
}
Detergent-resistant membrane (DRM) fractions enriched in ergosterol and sphingolipids can be isolated from yeast cells and have been proposed to represent the biochemical equivalents of lipid rafts. Most yeast plasma membrane proteins studied for their detergent solubility have been found in DRMs, except for the Hxt1 and Gap1 permeases. We here compared Gap1 detergent solubility in wild-type and various mutant cells under conditions promoting cell surface accumulation or ubiquitin-dependent… CONTINUE READING