Association of tissue factor activity with the surface of cultured cells.

@article{Maynard1975AssociationOT,
  title={Association of tissue factor activity with the surface of cultured cells.},
  author={J R Maynard and Carol A. Heckman and Frances Pitlick and Yale Nemerson},
  journal={The Journal of clinical investigation},
  year={1975},
  volume={55 4},
  pages={814-24}
}
Tissue factor occurs in a dormant state on the surface of cultured normal human fibroblasts and WISH 1 amnion cells. The activity of undisturbed monolayers or cells lifted with brief trypsin treatment (0.125 per cent trypsin for 1 min) increases up to 60-fold upon prolonged digestion with dilute trypsin (0.0025 per cent trypsin for 30 min); activity appears… CONTINUE READING