Association of the Chloroplastic Respiratory and Photosynthetic Electron Transport Chains of Chlamydomonas reinhardii with Photoreduction and the Oxyhydrogen Reaction.

  title={Association of the Chloroplastic Respiratory and Photosynthetic Electron Transport Chains of Chlamydomonas reinhardii with Photoreduction and the Oxyhydrogen Reaction.},
  author={T. Maione and M. Gibbs},
  journal={Plant physiology},
  volume={80 2},
The hydrogenase-dependent processes, photoreduction and the dark oxyhydrogen reaction, both of which can support CO(2) assimilation, were compared with aerobic photosynthesis and respiration for their sensitivity to electron transport inhibitors in cells and intact chloroplasts of Chlamydomonas reinhardii 11-32/6. Photoreduction but not photosynthesis was inhibited in chloroplasts and the oxyhydrogen reaction detected only in cells was inhibited up to 75 and 90%, respectively, by 150 micromolar… Expand
Characterization of an Electron Transport Pathway Associated with Glucose and Fructose Respiration in the Intact Chloroplasts of Chlamydomonas reinhardtii and Spinach.
Evidence is interpreted for a respiratory electron transport pathway functioning in the darkened, isolated chloroplast of darkened spinach and Chlamydomonas reinhardtii cell. Expand
Photoregulation of Fructose and Glucose Respiration in the Intact Chloroplasts of Chlamydomonas reinhardtii F-60 and Spinach
Of the four enzymes in the pathway catalyzing the release of CO2 from fructose, only glucose-6-P dehydrogenase was deactivated by light and by dithiothreitol, which inhibited release in both darkened chloroplasts. Expand
Respiratory control over photosynthetic electron transport in chloroplasts of higher-plant cells: evidence for chlororespiration
Flash-induced primary charge separation, detected as electrochromic absorbance change, the operation of the cytochrome b/f complex and the redox state of the plastoquinone pool were measured inExpand
Oxygen-exchange studies in Chlamydomonas mutants deficient in photosynthetic electron transport: Evidence for a Photosystem II-dependent oxygen uptake in vivo
It is concluded that in the absence of active Photosystem I, a permanent electron flow can occur in the light from Photosystem II to molecular O2, and it is proposed that an energy-dependent reverse electron transfer similar to that of Rhodospirillaceae might occurs in the chloroplast of Chlamydomonas. Expand
Coupling of Carbon Dioxide Fixation to the Oxyhydrogen Reaction in the Isolated Chloroplast of Chlamydomonas reinhardtii.
The oxyhydrogen reaction in the isolated Chlamydomonas reinhardtii chloroplast was studied and decrease in CO(2) uptake by dl-glyceraldehyde was taken to indicate that the regenerative phase and complete Calvin cycle turnover were involved. Expand
The hydrogen metabolism of sulfur deprived Chlamydomonas reinhardtii cells involves hydrogen uptake activities
It is shown that H 2 uptake occurs after prolonged incubation in the light as well as in sudden darkness, and can be delayed adding the phosphoribulose kinase inhibitor glycolaldehyde, suggesting a connection to carbohydrate metabolism. Expand
Photosynthetic H2 metabolism in Chlamydomonas reinhardtii (unicellular green algae)
Recent advances on green algal hydrogen metabolism are summarized and approaches discussed are beginning to address the biochemistry of anaerobic H2 photoproduction, its genes, proteins, regulation, and communication with other metabolic pathways in microalgae. Expand
Sustained photobiological hydrogen gas production upon reversible inactivation of oxygen evolution in the green alga Chlamydomonas reinhardtii.
The work describes a novel approach for sustained photobiological production of H(2) gas via the reversible hydrogenase pathway in the green alga Chlamydomonas reinhardtii, and suggests that photoreduction of ferredoxin is followed by electron donation to theversible hydrogenase. Expand
Control in the dark of the plastoquinone redox state by mitochondrial activity in Chlamydomonas reinhardtii
The results strongly suggest that alterations of the mitochondrial oxidative phosphorylations in the dark could induce an increase of the reduction state of the plastoquinone pool and a redistribution of the antennae between the two photosystems. Expand
Chlamydomonas: Hydrogenase and Hydrogen Production
An important aspect of Chlamydomonas reinhardtii’s metabolism is its ability to produce molecular hydrogen (H2) from protons and electrons. Hydrogen production is catalysed by twoExpand


Hydrogenase-Mediated Activities in Isolated Chloroplasts of Chlamydomonas reinhardii.
Isolated intact chloroplasts of Chlamydomonas reinhardii were found to catalyze photoreduction of CO(2) in the presence of 3-(3,4-dichlorophenyl)-1,1-dimethylurea when adapted under an atmosphere ofExpand
Hydrogen Metabolism in Photosynthetic Organisms, the Mechanism of Hydrogen Photoevolution
Studies utilizing cell-free systems and dealing with H2 photoevolution have been few. Abeles (1) reported H2 formation from reduced pyridine nucleotides in the presence of DCMU with a preparation ofExpand
Evidence for a respiratory chain in the chloroplast.
  • P. Bennoun
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1982
Chlororespiration is shown that, in dark-adapted chloroplasts, an electrochemical gradient is built up across the thylakoid membrane by transfer of electrons through the chlororespiratory chain as well as by reverse functioning of the chloroplast ATPases. Expand
  • H. Gaffron
  • Chemistry, Medicine
  • The Journal of general physiology
  • 1942
The oxyhydrogen reaction proceeds undisturbed only in the presence of carbon dioxide, which simultaneously is reduced according to the equation CO2 + 2H2 → H2O + (CH2O) = (carbohydrate). Expand
The kinetics of methyl viologen oxidation and reduction by the hydrogenase from Clostridium pasteurianum.
A mechanism for the reduction and oxidation of methyl viologen by Clostridium pasteurianum hydrogenase (hydrogen:ferredoxin oxidoreductase, EC 1.12.1) is proposed, and data are consistent with a mechanism in which the H2 and one methyl viOLOGen bind (either in order or randomly) with subsequent reduction and release of the methyl viology. Expand
Kinetics of the Oxyhydrogen Reaction in the Presence and Absence of Carbon Dioxide in Scenedesmus obliquus.
It was concluded that the oxyhydrogen reaction both in the presence and absence of CO(2) has properties in common with components of respiration and photosynthesis. Expand
Evidence for a membrane bound NADPHplastoquinone oxidoreductase in Chiamydomonas reinharadii
  • Arch Microbiol
  • 1982