Association of rasGAPSH3 binding protein 1, G3BP1, and rasGap120 with integrin containing complexes induced by an adhesion blocking antibody.

@article{Meng2004AssociationOR,
  title={Association of rasGAPSH3 binding protein 1, G3BP1, and rasGap120 with integrin containing complexes induced by an adhesion blocking antibody.},
  author={Xiaobo Meng and Jaykumar Krishnan and Yemen She and Werner E Ens and Kenneth G. Standing and John A. Wilkins},
  journal={Journal of proteome research},
  year={2004},
  volume={3 3},
  pages={506-16}
}
The adhesion blocking antibody 3S3 was used to probe the regulation of alpha5beta1 integrin mediated adhesion in K562 cells. This antibody prevented cellular adherence but it did not interfere with ligand binding by cells or purified integrin. Interaction with 3S3 induced change in the cytoskeletal organization resulting in extensive filopodia formation. The antibody also prevented ligand and anti-integrin antibody induced phosphorylation of FAK in a trans acting fashion. MS based analysis of… CONTINUE READING