Association of high-molecular-weight proteins with microtubules and their role in microtubule assembly in vitro.

@article{Murphy1975AssociationOH,
  title={Association of high-molecular-weight proteins with microtubules and their role in microtubule assembly in vitro.},
  author={Douglas B. Murphy and Gary G. Borisy},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1975},
  volume={72 7},
  pages={2696-700}
}
High-molecular-weight components (HMW) specifically associated with microtubule protein purified from porcine brain tissue were separated from tubulin by DEAE-Sephadex ion exchange chromatography. Analysis by viscometry, sedimentation, and electron microscopy of the unfractionated microtubule protein, separated HMW and tubulin fractions, and reconstituted mixtures showed that HMW promoted formation of ring structures at 5 degrees and tubule polymerization at 37 degrees. The HMW reassociated… CONTINUE READING