Association of enzymes with rat liver glycogen isolated by rate-zonal centrifugation.

@article{Barber1967AssociationOE,
  title={Association of enzymes with rat liver glycogen isolated by rate-zonal centrifugation.},
  author={Albert A. Barber and S A Orrell and Ernest Bueding},
  journal={The Journal of biological chemistry},
  year={1967},
  volume={242 18},
  pages={4040-4}
}
1. Rat liver glycogen was separated into fractions of relatively uniform particle sizes by large volume rate-zonal centrifugation of tissue homogenates with the use of sucrose density gradients. 2. After centrifugation, the distribution curve obtained by plotting the glycogen content of all collected fractions exhibited its maximum a short centrifugal distance from the zone which contained the starting sample. The curve then decreased progressively until an abrupt increase was noted in the zone… CONTINUE READING
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