Association between embryonic loss and damage to the zona pellucida by invasive micromanipulation during oviductal transfer of early-stage embryos in pigs.

@article{Ueno2007AssociationBE,
  title={Association between embryonic loss and damage to the zona pellucida by invasive micromanipulation during oviductal transfer of early-stage embryos in pigs.},
  author={Satoshi Ueno and Mayuko Kurome and Ryo Tomii and Katsumi Hiruma and Hitoshi Saitoh and Hiroshi Nagashima},
  journal={The Journal of reproduction and development},
  year={2007},
  volume={53 5},
  pages={1113-8}
}
The aim of this study was to examine the impact of zona pellucida damage, which might arise during somatic cell nuclear transfer (SCNT), on the development and survival of transferred embryos. The zonae pellucidae of in vitro matured oocytes were either punctured with 8- to 10-microm square-ended nuclear injection pipettes and piezo pulses or slit with 35- to 40-microm enucleation pipettes. Intact oocytes were used as controls. These oocytes were electroactivated to induce parthenogenesis and… CONTINUE READING
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