Assignment1 of fibroblast growth factor 18 (FGF18) to human chromosome 5q34 by use of radiation hybrid mapping and fluorescence in situ hybridization

  title={Assignment1 of fibroblast growth factor 18 (FGF18) to human chromosome 5q34 by use of radiation hybrid mapping and fluorescence in situ hybridization},
  author={Theodore E. Whitmore and M. F. Maurer and Shannon Sexson and Fenella C. Raymond and D C Conklin and Theresa A. Deisher},
  journal={Cytogenetic and Genome Research},
  pages={231 - 233}
FGF18 is a recently discovered member of the fibroblast growth factor family (Deisher et al., 1999). FGF18 has been reported to induce hepatic and intestinal proliferation in vivo (Hu et al., 1998), and to activate neural cell proliferation in vitro (Ohbayashi et al., 1998). Recently, FGF18 was mapped to both human chromosome 14p11 (Hu et al., 1999), and chromosome 5 (Sanger Centre, NCBI GeneMap’99). To help resolve this discrepancy, we carried out radiation hybrid mapping using both the… 

Figures from this paper

Fibroblast growth factors
A subset of the FGF family, expressed in adult tissue, is important for neuronal signal transduction in the central and peripheral nervous systems.
Fibroblast growth factor-18 is a trophic factor for mature chondrocytes and their progenitors.
Exposure of Fgf18 and the genes for two of its receptors in chondrocytes suggests that F gf18 may play an autocrine role in the biology of normal articular cartilage and demonstrates that FGF18 can act as a trophic factor for elastic chond rocytes and their progenitors in vivo and articular chondROcytes cultured in vitro.
Use of Adenovirus-Mediated Gene Transfer to Facilitate Biological Annotation of Novel Genes
Examples of two studies in which activities of novel growth factors were initially characterized using adenovirus-mediated gene transfer approach are presented to illustrate the potential of in vivo gene delivery approaches to facilitate functional analysis and focus of secondary investigation in a large screening effort.
A review of FGF18: Its expression, signaling pathways and possible functions during embryogenesis and post-natal development.
Although there remains much to be discovered and investigated on the functions and mechanisms of FGF18, it may play a role as a useful therapeutic target for various applications.
Zebrafish fgf24 functions with fgf8 to promote posterior mesodermal development
Both phenotypic and genetic evidence is provided that these Fgf signaling components interact with no tail and spadetail, two zebrafish T-box transcription factors that are required for the development of all posterior mesoderm.
Fibroblast Growth Factor 18 Increases the Trophic Effects of Bone Marrow Mesenchymal Stem Cells on Chondrocytes Isolated from Late Stage Osteoarthritic Patients
FGF18 can restore the responsiveness of OA chondrocytes to the trophic effects of MSCs and may be a good alternative cell source for regenerating cartilage tissue that is degraded during OA pathological changes.
Fibroblast Growth Factor-18 Reduced Infarct Volumes and Behavioral Deficits After Transient Occlusion of the Middle Cerebral Artery in Rats
It is demonstrated that FGF18 is an effective neuroprotective agent in a rat model of transient MCAo and was more efficacious than FGF2 on virtually all measures examined.
Sprifermin: Effects on Cartilage Homeostasis and Therapeutic Prospects in Cartilage-Related Diseases
Sprifermin (recombinant human FGF18, rhFGF18) is an effective DMOAD, which can not only promote the proliferation of articular chondrocyte and the synthesis of extracellular matrix, increase the thickness of cartilage in a dose-dependent manner, but also inhibit the activity of proteolytic enzymes and remarkedly slow down the degeneration of Cartilage.


Human fibroblast growth factor-18 stimulates fibroblast cell proliferation and is mapped to chromosome 14p11
It is shown that human FGF-18 was expressed primarily in the heart, skeletal muscle, and pancreas, and at lower levels in the other tissues, and was also expressed at low levels in certain cancer cell lines.
Structure and Expression of the mRNA Encoding a Novel Fibroblast Growth Factor, FGF-18*
The present results indicate that FGF-18 is a unique secreted signaling molecule in the adult lung and developing tissues.
FGF-18, a Novel Member of the Fibroblast Growth Factor Family, Stimulates Hepatic and Intestinal Proliferation
Cloning a novel member of the FGF family that is expressed primarily in the lungs and kidneys and at lower levels in the heart, testes, spleen, skeletal muscle, and brain suggests that FGF-18 is a pleiotropic growth factor that stimulates proliferation in a number of tissues.
High-resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones.
The results demonstrate the feasibility of rapidly producing high-resolution maps of human chromosomes by in situ hybridization and show that by hybridizing three or more cosmids simultaneously, gene order on the chromosome could be established unequivocally.
Cytogenetic and molecular delineation of the smallest commonly deleted region of chromosome 5 in malignant myeloid diseases.
A cytogenetic map of 5q31, together with the molecular characterization of the critical region, will facilitate the identification of a putative tumor-suppressor gene in this band and help identify patients with acute myeloid leukemia who did not have abnormalities of chromosome 5.
Location of ribosomal DNA in the human chromosome complement.
Hybridization of (3)H-labeled ribosomal RNA to human chromosomes on slides resulted in specific labeling of the satellite regions of chromosomes 13, 14, 15, 21, and 22, with an over-all efficiency of
Stanniocalcin 2: characterization of the protein and its localization to human pancreatic alpha cells.
Northern blot analysis revealed that the primary site of human STC2 production is the pancreas, and immunostaining localized theSTC2 protein to a subpopulation of cells in the islet, suggesting that STC 2 may play a role in glucose homeostasis.
International System for Human Cytogenetic Nomenclature
  • Iscn
  • Political Science
  • 1978
An exceptional reading e-book entitled International System For Human Cytogenetic Nomenclature provides a thorough legal analysis and guidance to state authorities, human rights and humanitarian actors and others.
Novel FGF Homologs
  • U.S. Patent No
  • 1999
ISCN ( 1985 ) . An International System for Human Cytogenetic Nomenclature , Harden DG , Klinger HP