Assignment of tra cistrons to EcoRI fragments of F sex factor DNA.

@article{Achtman1978AssignmentOT,
  title={Assignment of tra cistrons to EcoRI fragments of F sex factor DNA.},
  author={Mark Achtman and Ronald A. Skurray and Russell J. Thompson and Reiner Helmuth and S Hall and Lothar Beutin and Alvin J. Clark},
  journal={Journal of bacteriology},
  year={1978},
  volume={133 3},
  pages={1383-92}
}
We describe here the cloning of single EcoRI fragments from the tra region of F DNA using ColE1::Tn3 as vector. These plasmids, as well as the series of Skurray et al. (Proc. Natl. Acad. Sci. U.S.A. 73:64-68, 1976), have been used to refine the map positions of tra cistrons on the F factor as well as to define a new DNA transfer cistron, traM. The current map of the tra cistrons is presented. None of the known tra cistrons, with the exception of traG, straddles an EcoRI site. The EcoRI site at… CONTINUE READING

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 22 extracted citations

Characterisation of a λ transducing phage carrying the F conjugation gene traG

Molecular and General Genetics MGG • 2004
View 4 Excerpts
Highly Influenced

Investigations of the F conjugation gene traI:traI mutants and λtraI transducing phages

Molecular and General Genetics MGG • 2004
View 4 Excerpts
Highly Influenced

Characterization of the F plasmid bifunctional conjugation gene, traG

Molecular and General Genetics MGG • 1992
View 4 Excerpts
Highly Influenced

Similar Papers

Loading similar papers…