Assignment of the gene encoding the human thyrotropin-releasing hormone receptor to 8q23 by fluorescence in situ hybridization

  title={Assignment of the gene encoding the human thyrotropin-releasing hormone receptor to 8q23 by fluorescence in situ hybridization},
  author={N. Morrison and Sarah M. Duthie and E. Boyd and Karin Ann Eidne and J. Michael Connor},
  journal={Human Genetics},
A cDNA for human thyrotropin-releasing hormone (TRH) receptor has been isolated from a human pituitary cDNA library. By using this cDNA as a biotinylated probe, the gene encoding the TRH receptor has been localized to chromosome 8q23 by in situ hybridization. 

The computer modelling of human TRH receptor, TRH and TRH-like peptides.

This work verified the possibility of interactions between the human TRH receptor and TRH-like peptides presented in the prostate gland and found that polar amino acids belonging to the extracellular terminus of hTRHr transmembrane regions can participate in interactions between TRH and h TRHr.

TRH-like peptides.

Work deals with the biological function of TRH-like peptides in different parts of organisms where various mechanisms may serve for realisation of biological function, the role of pEEPam such as fertilization-promoting peptide, the mechanism influencing the proliferative ability of prostatic tissues, the neuroprotective and antidepressant function ofTRH- like peptide in brain and the regulation of thyroid status.

The role of Thyrotropin Releasing Hormone in aging and neurodegenerative diseases.

An overview of some of the roles of TRH, outside of the HPT axis, associated with pathological aging and neurodegeneration are provided and the potential ofTRH and TRH analogs for the treatment of Neurodegenerative diseases is discussed.

Genetic aspects of central hypothyroidism

  • R. Collu
  • Biology, Medicine
    Journal of endocrinological investigation
  • 2000
This review will analyze reports of mutations that affect the HPTA and result in either isolated central hypothyroidism or in the syndrome of combined pituitary hormone deficiency (CPHD).

TRH-like Peptides-Review

TRH-like peptides are characterized by substitution of basic amino acid histidine with neutral or acidic amino acid, like glutamic acid, phenylalanine, glutamine, tyrosine, leucin, valin, aspartic acid and asparagine.

FISHing chromosomes in endocrinology

Fluorescencein situ hybridization may shed light on the cytogenetic background and chromosomal alterations in the field of endocrinology, resulting in a better understanding of functional activities and various endocrine disorders.

Molecular cytogenetics : its use in human gene mapping and the detection and definition of subtle chromosomal aberrations

This work involved the investigation of three different molecular cytogenetic approaches, Fluorescence In Situ Hybridisation (FISH), Comparative Genomic Hybridisation (CGH) and Primed In Situ (PRINS)

Evaluation of cloned cells, animal model, and ATRA sensitivity of human testicular yolk sac tumor

Biological characteristics of cloned TYST cells were similar to the yolk sac tumor and differentiated from the columnar to glandular-like or goblet cells-like cells and all-trans-retinoic acid had significantly inhibitory effects on cell proliferation.



Cloning and functional characterisation of the human TRH receptor

Expression cloning of a cDNA encoding the mouse pituitary thyrotropin-releasing hormone receptor.

Using Xenopus laevis oocytes in an expression cloning strategy, a cDNA clone is isolated that encodes the mouse pituitary TRH-R, a protein of 393 amino acids that shows similarities to other guanine nucleotide-binding regulatory protein-coupled receptors.

Cloning and expression of the thyrotropin-releasing hormone receptor from GH3 rat anterior pituitary cells.

Full functionality of the predicted 412-amino-acid receptor protein was demonstrated by functional expression of cell surface receptors in Xenopus oocytes after both cytoplasmic injection of sense RNA transcribed in vitro from this cDNA and nuclear injection of the cDNA under the control of the Herpes simplex virus thymidine kinase promoter.

Functional expression and molecular characterization of the thyrotrophin-releasing hormone receptor from the rat anterior pituitary gland.

The rat TRH-R protein showed considerable homology with that of the mouse, except for a deletion of 232 bp in the 3'-coding region, which did not appear to affect the functional characteristics of the receptor, as shown by electrophysiological studies with Xenopus oocytes and by transfection of the cDNA into COS-7 cells.

Novel non-isotopic in situ hybridization technique detects small (1 Kb) unique sequences in routinely G-banded human chromosomes: fine mapping of N-myc and beta-NGF genes.

A novel in situ hybridization technique is described that combines, for the first time, the high spacial resolution and rapid signal development of the non-isotopic approach with the previously unrivalled sensitivity of autoradiography.

Reverse chromosome painting: a method for the rapid analysis of aberrant chromosomes in clinical cytogenetics.

The usefulness of this method for routine clinical cytogenetics is shown by the analysis of cases involving an insertion, a deletion, a translocation, and two cases of a chromosome with additional material of unknown origin.

Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization.

The use of fluorescence in situ hybridization for chromosome classification and detection of chromosome aberrations is described and chromosomes in human-hamster hybrid cell lines were intensely and uniformly stained in metaphase spreads and interphase nuclei when human genomic DNA was used as a probe.