Assignment of the gene encoding the human thyrotropin-releasing hormone receptor to 8q23 by fluorescence in situ hybridization

  title={Assignment of the gene encoding the human thyrotropin-releasing hormone receptor to 8q23 by fluorescence in situ hybridization},
  author={N. Morrison and S. Duthie and E. Boyd and K. Eidne and J. Connor},
  journal={Human Genetics},
A cDNA for human thyrotropin-releasing hormone (TRH) receptor has been isolated from a human pituitary cDNA library. By using this cDNA as a biotinylated probe, the gene encoding the TRH receptor has been localized to chromosome 8q23 by in situ hybridization. 
The computer modelling of human TRH receptor, TRH and TRH-like peptides.
This work verified the possibility of interactions between the human TRH receptor and TRH-like peptides presented in the prostate gland and found that polar amino acids belonging to the extracellular terminus of hTRHr transmembrane regions can participate in interactions between TRH and h TRHr. Expand
TRH-like peptides.
Work deals with the biological function of TRH-like peptides in different parts of organisms where various mechanisms may serve for realisation of biological function, the role of pEEPam such as fertilization-promoting peptide, the mechanism influencing the proliferative ability of prostatic tissues, the neuroprotective and antidepressant function ofTRH- like peptide in brain and the regulation of thyroid status. Expand
The role of Thyrotropin Releasing Hormone in aging and neurodegenerative diseases.
An overview of some of the roles of TRH, outside of the HPT axis, associated with pathological aging and neurodegeneration are provided and the potential ofTRH and TRH analogs for the treatment of Neurodegenerative diseases is discussed. Expand
Genetic aspects of central hypothyroidism
  • R. Collu
  • Medicine
  • Journal of endocrinological investigation
  • 2000
This review will analyze reports of mutations that affect the HPTA and result in either isolated central hypothyroidism or in the syndrome of combined pituitary hormone deficiency (CPHD). Expand
TRH-like Peptides-Review
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FISHing chromosomes in endocrinology
Fluorescencein situ hybridization may shed light on the cytogenetic background and chromosomal alterations in the field of endocrinology, resulting in a better understanding of functional activities and various endocrine disorders. Expand
Comparative analysis of the mouse and human genes (Matn2 and MATN2) for matrilin-2, a filament-forming protein widely distributed in extracellular matrices.
  • L. Mátés, É. Korpos, +4 authors I. Kiss
  • Biology, Medicine
  • Matrix biology : journal of the International Society for Matrix Biology
  • 2002
The genomic organization of the highly conserved mouse and human MATN2 loci, which cover >100 kb and 167.167 kb genomic regions, respectively, are presented and it is revealed that alternative transcripts with identical protein coding regions are transcribed from two promoters in both species. Expand
Molecular cytogenetics : its use in human gene mapping and the detection and definition of subtle chromosomal aberrations
This work involved the investigation of three different molecular cytogenetic approaches, Fluorescence In Situ Hybridisation (FISH), Comparative Genomic Hybridisation (CGH) and Primed In Situ (PRINS)Expand
Evaluation of cloned cells, animal model, and ATRA sensitivity of human testicular yolk sac tumor
Biological characteristics of cloned TYST cells were similar to the yolk sac tumor and differentiated from the columnar to glandular-like or goblet cells-like cells and all-trans-retinoic acid had significantly inhibitory effects on cell proliferation. Expand


Two isoforms of the thyrotropin-releasing hormone receptor generated by alternative splicing have indistinguishable functional properties.
Functional expression in Xenopus laevis oocytes indicates that both cDNAs encode fully functional TRH receptors, and the perfect colinearity between genomic DNA and TRH-R(412) cDNA demonstrates that no other introns are present within the coding region of the TRH receptor gene. Expand
Cloning and functional characterisation of the human TRH receptor
A cDNA encoding the human pituitary TRH receptor (TRH-R) protein is isolated with a predicted amino acid sequence of 398 amino acids and it is believed that the COOH terminal region of this family of receptors may play an important role in receptor downregulation/internalisation, and possibly G-protein coupling. Expand
Expression cloning of a cDNA encoding the mouse pituitary thyrotropin-releasing hormone receptor.
Using Xenopus laevis oocytes in an expression cloning strategy, a cDNA clone is isolated that encodes the mouse pituitary TRH-R, a protein of 393 amino acids that shows similarities to other guanine nucleotide-binding regulatory protein-coupled receptors. Expand
Distribution of thyrotrophin-releasing hormone receptor messenger RNA in rat pituitary and brain
This study has shown for the first time the exact site of thyrotrophin-releasing hormone receptor expression in the central nervous system and these results correlate well with regions thought to possess thyrotrophic hormone recognition sites. Expand
Cloning and expression of the thyrotropin-releasing hormone receptor from GH3 rat anterior pituitary cells.
Full functionality of the predicted 412-amino-acid receptor protein was demonstrated by functional expression of cell surface receptors in Xenopus oocytes after both cytoplasmic injection of sense RNA transcribed in vitro from this cDNA and nuclear injection of the cDNA under the control of the Herpes simplex virus thymidine kinase promoter. Expand
Functional expression and molecular characterization of the thyrotrophin-releasing hormone receptor from the rat anterior pituitary gland.
The rat TRH-R protein showed considerable homology with that of the mouse, except for a deletion of 232 bp in the 3'-coding region, which did not appear to affect the functional characteristics of the receptor, as shown by electrophysiological studies with Xenopus oocytes and by transfection of the cDNA into COS-7 cells. Expand
Chromosomal assignment of a glutamic acid transfer RNA (tRNAGlu) gene to 1p36
In fibroblasts from gorilla (Gorilla gorilla) using biotin labelling, a single site of hybridisation occurred at 1qter which provides further support for homology of 1q in the higher apes and human 1p. Expand
Diverse roles of thyrotropin-releasing hormone in brain, pituitary and spinal function
Abstract Regulatory peptides are increasingly gaining prominence and importance as potential transmitter and homeostatic agents in the vertebrate CNS. The tripeptide, thyrotropin-releasing hormoneExpand
Distribution of thyrotropin-releasing hormone binding sites: autoradiographic study in infant and adult human hippocampal formation
The rostrocaudal distribution of thyrotropin-releasing hormone (TRH) binding sites was studied in the human hippocampus and a single class of high affinity [3H]MeTRH binding sites with an apparent dissociation constant in the nanomolar range has been shown both in the infant and the adult. Expand
Novel non-isotopic in situ hybridization technique detects small (1 Kb) unique sequences in routinely G-banded human chromosomes: fine mapping of N-myc and beta-NGF genes.
A novel in situ hybridization technique is described that combines, for the first time, the high spacial resolution and rapid signal development of the non-isotopic approach with the previously unrivalled sensitivity of autoradiography. Expand