Assignment of the gene encoding the human thyrotropin-releasing hormone receptor to 8q23 by fluorescence in situ hybridization

@article{Morrison2004AssignmentOT,
  title={Assignment of the gene encoding the human thyrotropin-releasing hormone receptor to 8q23 by fluorescence in situ hybridization},
  author={N. Morrison and Sarah M. Duthie and E. Boyd and Karin Ann Eidne and J. Michael Connor},
  journal={Human Genetics},
  year={2004},
  volume={93},
  pages={716-718}
}
A cDNA for human thyrotropin-releasing hormone (TRH) receptor has been isolated from a human pituitary cDNA library. By using this cDNA as a biotinylated probe, the gene encoding the TRH receptor has been localized to chromosome 8q23 by in situ hybridization. 

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Cloning and functional characterisation of the human TRH receptor

Expression cloning of a cDNA encoding the mouse pituitary thyrotropin-releasing hormone receptor.

TLDR
Using Xenopus laevis oocytes in an expression cloning strategy, a cDNA clone is isolated that encodes the mouse pituitary TRH-R, a protein of 393 amino acids that shows similarities to other guanine nucleotide-binding regulatory protein-coupled receptors.

Cloning and expression of the thyrotropin-releasing hormone receptor from GH3 rat anterior pituitary cells.

TLDR
Full functionality of the predicted 412-amino-acid receptor protein was demonstrated by functional expression of cell surface receptors in Xenopus oocytes after both cytoplasmic injection of sense RNA transcribed in vitro from this cDNA and nuclear injection of the cDNA under the control of the Herpes simplex virus thymidine kinase promoter.

Functional expression and molecular characterization of the thyrotrophin-releasing hormone receptor from the rat anterior pituitary gland.

TLDR
The rat TRH-R protein showed considerable homology with that of the mouse, except for a deletion of 232 bp in the 3'-coding region, which did not appear to affect the functional characteristics of the receptor, as shown by electrophysiological studies with Xenopus oocytes and by transfection of the cDNA into COS-7 cells.

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TLDR
A novel in situ hybridization technique is described that combines, for the first time, the high spacial resolution and rapid signal development of the non-isotopic approach with the previously unrivalled sensitivity of autoradiography.

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TLDR
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TLDR
The use of fluorescence in situ hybridization for chromosome classification and detection of chromosome aberrations is described and chromosomes in human-hamster hybrid cell lines were intensely and uniformly stained in metaphase spreads and interphase nuclei when human genomic DNA was used as a probe.