Assignment of the gene encoding the catalytic subunit Cβ of CAMP-dependent protein kinase to the p36 band on chromosome

@article{Simard2005AssignmentOT,
  title={Assignment of the gene encoding the catalytic subunit C$\beta$ of CAMP-dependent protein kinase to the p36 band on chromosome},
  author={Jacques Simard and Dominique B{\'e}rub{\'e} and M{\aa}rten Sandberg and Karl Heinz Grzeschik and Richard Gagn{\'e} and Vidar Hansson and Tore Jahnsen},
  journal={Human Genetics},
  year={2005},
  volume={88},
  pages={653-657}
}
SummaryA cDNA for the human catalytic subunit (Cβ) of cAMP-dependent protein kinase (PKA) has been cloned from a testis cDNA library. In the present study, we have determined the chromosomal localization of this gene using a cDNA for Cβ as a probe. Southern blot analysis of genomic DNA from human/mouse cell hybrids revealed that the presence or absence of a 20-kbXbaI fragment, which hybridized with the Cβ probe, was concordant with the presence of human chromosome 1.In situ hybridization to… 

Mapping of the regulatory type Iα and catalytic β subunits of cAMP-dependent protein kinase and interleukin 1α and 1β in the pig

TLDR
The genes coding for the regulatory type Iα subunit (PRKAR1A) and the catalytic β subunit of cAMP-dependent protein kinase and the genes for interleukin 1α (IL1A and IL1B) were localized in the pig by means of radioactive in situ hybridization.

Genomic Expression Discovery Predicts Pathways and Opposing Functions behind Phenotypes*

TLDR
Meningiomas appear to acquire aberrant phenotypes by disturbing the balanced expression of molecules that promote opposing functions and a role of genomic expression discovery in functional genomics of living systems is proposed.

High frequency of T9 and CFTR mutations in children with idiopathic bronchiectasis

TLDR
The gene responsible for cystic fibrosis, the CFTR, was shown to have a role in the manifestation of IB, as gene mutations and a significantly high proportion of allele T5 of the polythymidine tract (Tn) in intron 8 (IVS8) have been observed in patients.

Contrôle hormonal de la stéroïdogenèse et tumorigenèse cortico-surrénalienne : utilisation de la trangenèse chez la souris pour le développement de nouvelles lignées cellulaires et de modèles animaux de pathologies tumorales par oncogenèse ciblée

TLDR
Nous avons montre que l'expression dans la corticosurrenale de souris d'une sous unite regulatrice RIalpha tronquee de the PKA reproduit l'hyperactivite endocrine rencontree dans les hyperplasies micronodulaires pigmentees des surrenales.

Inherited disposition to cardiac myxoma development

TLDR
Genetically engineered mutant Prkar1a mouse models show an increased propensity to develop tumours, and have established a role for R1α in initiating tumour formation and, potentially, in maintaining cell proliferation.

Cloning of a mouse protein kinase A catalytic subunit pseudogene and chromosomal mapping of C subunit isoforms

TLDR
A novel C-related sequence, Cx, is reported from a murine genomic library, which is 89.8% identical to part of the Cα coding region, but lacks all of the introns present in this gene, suggesting that is arose via retroposition.

Localization of the IGHG, PRKACB, and TNP2 genes in pigs by in situ hybridization

TLDR
The results show that the IGHG locus in pigs, similar to the situation in other mammalian species, is located on the terminal region of the chromosome.

References

SHOWING 1-10 OF 45 REFERENCES

Isolation of cDNA clones coding for the catalytic subunit of mouse cAMP-dependent protein kinase.

TLDR
Southern blot analysis of total genomic DNA suggests that there is a single mouse gene coding for the C subunit of cAMP-dependent protein kinase, and enriched mRNA preparation was used to prepare and differentially screen a cDNA library.

Multiple mRNA species code for the catalytic subunit of the cAMP-dependent protein kinase from LLC-PK1 cells. Evidence for two forms of the catalytic subunit.

TLDR
Evidence for the existence of two forms of the catalytic (C) subunit of the cAMP-dependent protein kinase is presented and at least two distinct genes encode the C subunit which are expressed in LLC-PK1 cells.

Isolation of a cDNA clone for the type I regulatory subunit of bovine cAMP-dependent protein kinase.

TLDR
A cDNA clone for the type I regulatory subunit (RI) of cAMP-dependent protein kinase (ATP:protein phosphotransferase, EC 2.7.37) was isolated from bovine testis by a differential screening method and a recombinant plasmid with a 1,550-nucleotide insert that selected through hybridization a mRNA coding for a 55,000-dalton protein that crossreacts with anti-RI antibodies is identified.

Molecular cloning of a tissue-specific protein kinase (C gamma) from human testis--representing a third isoform for the catalytic subunit of cAMP-dependent protein kinase.

TLDR
The molecular cloning of a third isoform of C, from a human testis cDNA library, as well as the isolation of human cDNAs for C alpha and C beta are reported, suggesting that C gamma may be distinct in its protein substrate specificity or its interaction with the different regulatory subunits.

Genetic characterization of a brain-specific form of the type I regulatory subunit of cAMP-dependent protein kinase.

TLDR
The native form of RI beta in brain could also be distinguished from RI alpha by its abnormal migration on NaDodSO4/PAGE, and RI beta protein produced in 3T3 cells was shown to be functional by its ability to form a cAMP-dependent holoenzyme with the catalytic subunit.

The molecular cloning of a type II regulatory subunit of the cAMP-dependent protein kinase from rat skeletal muscle and mouse brain.

  • J. ScottM. Glaccum E. Krebs
  • Biology, Chemistry
    Proceedings of the National Academy of Sciences of the United States of America
  • 1987
A cDNA clone for a type II regulatory (R) subunit of the cAMP-dependent protein kinase (ATP:protein phosphotransferase, EC 2.7.1.37) was isolated from a rat skeletal muscle library using a specific

Assignment of a structural gene forβ-glucuronidase to human chromosome C7

TLDR
An electrophoretic technique was developed which allows the separation of GUS from the enzyme present in cultured murine, Chinese and Syrian hamster cells in one buffer system on Cellogel, and results indicate that a structural gene for human β-glucuronidase is located on chromosome C7.

Genomic organization of the human asparagine transfer RNA genes: localization to the U1 RNA gene and class I pseudogene repeat units.

TLDR
It is proposed that the split into two distinct loci was facilitated by a pericentric chromosome inversion, and these two gene families are closely linked on repeat units of 180 kb in size, which are probably organized in tandem arrays.