Assignment of human thyrotropin-releasing hormone (TRH) receptor gene to chromosome 8

  title={Assignment of human thyrotropin-releasing hormone (TRH) receptor gene to chromosome 8},
  author={Masanobu Yamada and Tsuyoshi Monden and Syuntaro Konaka and Masatomo Mori},
  journal={Somatic Cell and Molecular Genetics},
The human gene encoding thyrotropin-releasing hormone receptor was assigned to chromosome 8, using human-Chinese hamster ovary somatic cell hybrids, analyzed by Southern hybridizations. Hybridization was carried out with a32P-labeled fragment of the human TRH-R genomic DNA. Hybridization of this probe to a human specific 10.5-kb DNA fragment of EcoRI-digested WBC DNA was used to localize the human TRH-R gene. No hybridization, by contrast, was seen with this probe and hamster DNA after EcoRI… 

The role of Thyrotropin Releasing Hormone in aging and neurodegenerative diseases.

An overview of some of the roles of TRH, outside of the HPT axis, associated with pathological aging and neurodegeneration are provided and the potential ofTRH and TRH analogs for the treatment of Neurodegenerative diseases is discussed.

Non-clinical pharmacokinetic profiles of rovatirelin, an orally available thyrotropin-releasing hormone analogue

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Human mass balance, pharmacokinetics and metabolism of rovatirelin and identification of its metabolic enzymes in vitro

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Human corticotropin releasing hormone gene is located on the long arm of chromosome 8.

The absence of secondary hybridization strongly suggests that hypothalamic and placental CRH are transcribed from the same gene.

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The sequence and functioning analyses showed the same expression of TRH-R mRNA in the pituitary adenomas of patients with acromegaly as that in the normal pituitaries, indicating that 2) the paradoxical response of GH secretion to TRH found in the patients might be a direct effect ofTRH on TRH -R expressed in the adenoma.

Expression cloning of a cDNA encoding the mouse pituitary thyrotropin-releasing hormone receptor.

Using Xenopus laevis oocytes in an expression cloning strategy, a cDNA clone is isolated that encodes the mouse pituitary TRH-R, a protein of 393 amino acids that shows similarities to other guanine nucleotide-binding regulatory protein-coupled receptors.

Molecular cloning of a complementary deoxyribonucleic acid encoding the thyrotropin-releasing hormone receptor and regulation of its messenger ribonucleic acid in rat GH cells.

It is concluded that the rat TRH receptor (rTRHR) shares high primary sequence similarity to the mouse thyrotrope TRHR, but the rTRHR has an extension of 19 amino acids at its carboxy-terminus, which is lacking in the mTRHR.

Isolation and characterization of the human gonadotropin-releasing hormone gene in the hypothalamus and placenta.

The GnRH gene has been cloned in several species, but the location of the promoter and the exact start of transcription have not previously been determined, and this characterization will allow hormonal regulatory studies to be performed using gene transfer techniques.

Linkage of theleuS, emtB, andchr genes on chromosome 5 in humans and expression of human genes encoding protein synthetic components in human-Chinese hamster hybrids

Results indicate that the ribosomal protein product of the human emtBgene is incorporated into functional ribosomes in place of the corresponding Chinese hamster protein, raising several interesting questions concerning the coordinate regulation of genes encoding ribosome proteins in mammalian cells.

Genetic counterselective procedure to isolate interspecific cell hybrids containing single human chromosomes: Construction of cell hybrids and recombinant DNA libraries specific for human chromosomes 3 and 4

Counterselection against genes on human chromosome 5 was applied to interspecific human-Chinese hamster cell hybrids which retained this and one additional human chromosome in order to generate cell


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