Assignment of congested NMR spectra: carbonyl backbone enrichment via the Entner-Doudoroff pathway.

@article{Goldbourt2007AssignmentOC,
  title={Assignment of congested NMR spectra: carbonyl backbone enrichment via the Entner-Doudoroff pathway.},
  author={Amir Goldbourt and Loren A. Day and Ann E McDermott},
  journal={Journal of magnetic resonance},
  year={2007},
  volume={189 2},
  pages={157-65}
}
In NMR spectra of complex proteins, sparse isotope enrichment can be important, in that the removal of many (13)C-(13)C homonuclear J-couplings can narrow the lines and thereby facilitate the process of spectral assignment and structure elucidation. We present a simple scheme for selective yet extensive isotopic enrichment applicable for production of proteins in organisms utilizing the Entner-Doudoroff (ED) metabolic pathway. An enrichment scheme so derived is demonstrated in the context of a… CONTINUE READING

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