Assignment-free solution NMR method reveals CesT as an unswapped homodimer.

@article{Rumpel2008AssignmentfreeSN,
  title={Assignment-free solution NMR method reveals CesT as an unswapped homodimer.},
  author={Sigrun Rumpel and Raghavendran Lakshmi and Stefan J. Becker and Markus Zweckstetter},
  journal={Protein science : a publication of the Protein Society},
  year={2008},
  volume={17 11},
  pages={
          2015-9
        }
}
The X-ray structure of the homodimeric chaperone CesT is the only structure among the type three secretion system (TTSS) chaperones that shows a domain swap. This swap has potential importance for the mechanism of effector translocation through a TTSS. Here we present two nuclear magnetic resonance strategies exploiting pre-existing structural models and residual dipolar couplings (RDCs), which reveal the unswapped 35.4-kDa dimer to be present in solution. Particularly efficient is the… CONTINUE READING

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