Assessment of three techniques for delivering stem cells to the heart using PET and MR imaging

  title={Assessment of three techniques for delivering stem cells to the heart using PET and MR imaging},
  author={Esmat Elhami and Bryson Dietz and Bo Xiang and Jixian Deng and Fei Wang and Chao Chi and Andrew L. Goertzen and Shadreck Mzengeza and Darren H. Freed and Rakesh C Arora and Ganghong Tian},
  journal={EJNMMI Research},
  pages={72 - 72}
BackgroundStem cell therapy has a promising potential for the curing of various degenerative diseases, including congestive heart failure (CHF). In this study, we determined the efficacy of different delivery methods for stem cell administration to the heart for the treatment of CHF. Both positron emission tomography (PET) and magnetic resonance imaging (MRI) were utilized to assess the distribution of delivered stem cells.MethodsAdipose-derived stem cells of male rats were labeled with super… 

Molecular imaging in stem cell-based therapies of cardiac diseases.

In Vivo Tracking of Cell Therapies for Cardiac Diseases with Nuclear Medicine

Radiopharmaceutical cell labeling, firstly developed to track leukocytes, has been used successfully to evaluate the migration of cell therapies for myocardial diseases and a substantial rise in the amount of reports employing this methodology has taken place in the previous years.

Cell therapy for ARDS: efficacy of endobronchial versus intravenous administration and biodistribution of MAPCs in a large animal model

The EB or intravenous routes of administration of MAPC are both effective for the treatment of ARDS in an acute sheep model, and the effect ofMAPC therapy is not dependent of parenchymal integration or systemic biodistribution.

Mechanisms behind stem cell therapy in acute myocardial infarction

Ischemic heart disease is one of the leading cause of death in the Western world. There is convincing evidence that stem cell therapy improves cardiac function and reduces the scar formation

Clinical imaging in regenerative medicine

Imaging studies in brain, heart and islets share a common set of challenges, including developing novel labeling approaches to improve detection thresholds and early delineation of toxicity and function, and addressing safety concerns associated with genetic labels.

PET Cell Tracking Using 18F-FLT is Not Limited by Local Reuptake of Free Radiotracer

A novel method of cell tracking using 3′-deoxy-3′-L-[18F]-fluorothymidine (18F-FLT) is assessed, which it is hypothesised will result in minimal local radiotracer reuptake and allow a more accurate estimation of cell retention.

Molecular imaging of stem cells for the treatment of acute myocardial infarction.

The mechanisms by which molecular imaging enables insights into and the development of stem cell therapy are illustrated.



SPECT and PET to optimize cardiac stem cell therapy

PET and SPECT imaging have the potential to play a major role in pre-clinical and clinical studies of stem cell therapies because they provide information on myocardial viability, ejection fraction, infarct size, coronary flow reserve, and myocardian perfusion (which are important endpoints following stem cell transplantation) in addition to permitting quantification of stem Cell engraftment.

Magnetic Resonance Imaging Overestimates Ferumoxide-Labeled Stem Cell Survival After Transplantation in the Heart

The persistence of significant iron-dependent MRI signal derived from ferumoxides-containing macrophages despite few or no viable stem cells 3 weeks after transplantation indicates that MRI of ferumoxide-labeled cells does not reliably report long-term stem cell engraftment in the heart.

Adipose-derived stem cells are an effective cell candidate for treatment of heart failure: an MR imaging study of rat hearts.

Results of this study suggest that ASCs were able to improve cardiac function of infarcted rat hearts and paracrine effect may be the mechanism underlying the improved cardiac function and increased capillary density.

Tissue distribution of 18F-FDG-labeled peripheral hematopoietic stem cells after intracoronary administration in patients with myocardial infarction.

It is shown that 18F-FDG-labeled stem cell PET could be used to assess the tissue distribution and to measure and optimize the amount of stem cells at a target tissue.

18F-FDG Labeling of Mesenchymal Stem Cells and Multipotent Adult Progenitor Cells for PET Imaging: Effects on Ultrastructure and Differentiation Capacity

Direct labeling of MSCs and MAPCs with 18F-FDG is a suitable technique to noninvasively assess cell delivery and early retention with PET and confirmed that the main cellular properties are not rigorously affected.

Viability and proliferation potential of adipose-derived stem cells following labeling with a positron-emitting radiotracer

The results indicate that ASCs can be safely labeled with FDG concentration up to 25 Bq/cell, without compromising their biological function, and developed a labeling protocol for labeling ASCs with a readily available PET tracer, FDG.

Imaging stem cells implanted in infarcted myocardium.

Myocardial substrate and route of administration determine acute cardiac retention and lung bio-distribution of cardiosphere-derived cells

Acute cardiac retention and lung bio-distribution vary with the myocardial substrate and injection route, and two-photon microscopy indicated that CDCs escaped to the lungs via the coronary veins following intra-myocardial injection.