Assessment of three generations of mice derived by ICSI using freeze-dried sperm.

@article{Li2009AssessmentOT,
  title={Assessment of three generations of mice derived by ICSI using freeze-dried sperm.},
  author={M W Li and Brandon J. Willis and Stephen M. Griffey and Jimmy L. Spearow and K. C. Kent Lloyd},
  journal={Zygote},
  year={2009},
  volume={17 3},
  pages={239-51}
}
Although the derivation of mice by intracytoplasmic sperm injection (ICSI) using freeze-dried sperm has been demonstrated previously, a comprehensive analysis of their viability, health, and fertility has not. The purpose of the present study was to determine the extent to which ICSI using freeze-dried sperm stored at 4 degrees C for 1-2 months from mice on either an inbred (C57BL/6J) or hybrid (B6D2F1/J) genetic background results in genomic instability and/or phenotypic abnormality in mice… CONTINUE READING