Assessment of the separation of X- and Y-bearing sperm on albumin gradients using double-label fluorescence in situ hybridization.

@article{Wang1994AssessmentOT,
  title={Assessment of the separation of X- and Y-bearing sperm on albumin gradients using double-label fluorescence in situ hybridization.},
  author={He Xiang Wang and Sean P. Flaherty and Nicholas J. Swann and Colin D. Matthews},
  journal={Fertility and sterility},
  year={1994},
  volume={61 4},
  pages={720-6}
}
OBJECTIVE To determine the ratio of X- to Y-bearing human spermatozoa in fractions isolated from discontinuous albumin gradients. DESIGN The proportions of X- and Y-bearing sperm were determined in neat semen samples (control) and in albumin-separated fractions from the same samples. Two albumin methods were used: a two-layer method (experiment 1) and a… CONTINUE READING