Assessment of the mosaic structure in the Helicobacter pylori cagA gene 3'-region using an improved polymerase chain reaction-based assay.

@article{Monstein2012AssessmentOT,
  title={Assessment of the mosaic structure in the Helicobacter pylori cagA gene 3'-region using an improved polymerase chain reaction-based assay.},
  author={H J Monstein and Anna Ryberg and Anneli Karlsson},
  journal={Diagnostic microbiology and infectious disease},
  year={2012},
  volume={73 3},
  pages={
          281-3
        }
}
The mosaic structure of the cagA gene has been suggested to affect Helicobacter pylori CagA-associated pathogenesis. An improved polymerase chain reaction assay allowed for a rapid and detailed molecular analysis of the cagA gene 3'-region in a single amplification step, followed by amplicon sequencing using universal M13 and T7 sequencing primers. 

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