Assessment of the cellular internalization of thermolytic phosphorothioate DNA oligonucleotide prodrugs.


The bioactivity of a CpG-containing phosphorothioate DNA oligonucleotide with thermolytic 2-(N-formyl-N-methylamino)ethyl (fma) thiophosphate groups in mice led us to investigate the parameters affecting the internalization of these thermosensitive DNA prodrugs in various cell lines. Flow cytometry and confocal microscopy analyses indicate that 5… (More)
DOI: 10.1016/j.bmc.2013.04.071


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