Assessment of the cell-mediated immune response in chickens by detection of chicken interferon-gamma in response to mitogen and recall Newcastle disease viral antigen stimulation.

@article{Lambrecht2004AssessmentOT,
  title={Assessment of the cell-mediated immune response in chickens by detection of chicken interferon-gamma in response to mitogen and recall Newcastle disease viral antigen stimulation.},
  author={B{\'e}n{\'e}dicte Lambrecht and Martine Gonze and Guy Meulemans and Thierry P van den Berg},
  journal={Avian pathology : journal of the W.V.P.A},
  year={2004},
  volume={33 3},
  pages={343-50}
}
The potential of a capture enzyme-linked immunosorbent assay (ELISA) specific for chicken interferon-gamma (ChIFN-gamma) has been evaluated as a tool to assess cell-mediated immunity (CMI) in the chicken. In a first step, ChIFN-gamma production and cell proliferation of mitogen-activated chicken splenocytes have been compared. In general, for each of the stimulation conditions where significant proliferation was observed, production of ChIFN-gamma could be measured by ELISA. In our hands, the… CONTINUE READING

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