Assessment of simple marker-free genetic transformation techniques in alfalfa

@article{Ferradini2011AssessmentOS,
  title={Assessment of simple marker-free genetic transformation techniques in alfalfa},
  author={Nicoletta Ferradini and Alessandro Nicolia and Stefano Capomaccio and Fabio Veronesi and Daniele Rosellini},
  journal={Plant Cell Reports},
  year={2011},
  volume={30},
  pages={1991-2000}
}
Methods to avoid the presence of selectable marker genes (SMG) in transgenic plants are available but not implemented in many crop species. We assessed the efficiency of simple marker-free Agrobacterium-mediated transformation techniques in alfalfa: regeneration without selection, or marker-less, and co-transformation with two vectors, one containing the SMG and one containing a non-selected gene. To easily estimate the efficiency of marker-less transformation, the nptII and the GUS markers… CONTINUE READING

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