Assessment of siRNA pharmacokinetics using ELISA-based quantification.


Here, we developed a novel ELISA-based assay for quantifying double-stranded intact siRNAs for in vivo pharmacokinetic analysis. The assay makes use of dual-labeled unmethylated or methylated siRNA, 5'-end-labeled on one strand with biotin (capture marker), and with dinitrophenol (detection marker), on the other end. This ELISA-based assay was linear over… (More)
DOI: 10.1016/j.jconrel.2009.12.004


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