Assessment of sample handling practices on microbial activity in sputum samples from patients with cystic fibrosis.

@article{Nelson2010AssessmentOS,
  title={Assessment of sample handling practices on microbial activity in sputum samples from patients with cystic fibrosis.},
  author={Andrew Nelson and Anthony De Soyza and Stephen J. Bourke and John David Perry and Stephen Paul Cummings},
  journal={Letters in applied microbiology},
  year={2010},
  volume={51 3},
  pages={272-7}
}
AIM The aim of this study was to quantitatively and qualitatively assess the effect of sample storage on the metabolically active microbial community found in sputum samples from patients with cystic fibrosis (CF). METHODS Sputum samples were collected and split in two equal aliquots one of which was immersed in RNAlater and refrigerated immediately, the second stored at room temperature for 24 h and RNAlater was subsequently added. mRNA was extracted, and RT-PCR-DGGE and qPCR analysis of the… CONTINUE READING

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