Assessment of real-time RT-PCR for quantification of Lactobacillus plantarum gene expression during stationary phase and nutrient starvation.

@article{Marco2008AssessmentOR,
  title={Assessment of real-time RT-PCR for quantification of Lactobacillus plantarum gene expression during stationary phase and nutrient starvation.},
  author={Maria L Marco and Michiel Kleerebezem},
  journal={Journal of applied microbiology},
  year={2008},
  volume={104 2},
  pages={587-94}
}
AIMS In this study, we evaluated the impact of different real-time reverse-transcription PCR (RT-PCR) data normalization methods on the interpretation of stationary-phase and nutrient-starved Lactobacillus plantarum WCFS1 gene expression levels. METHODS AND RESULTS Lactobacillus plantarum WCFS1 culture characteristics and housekeeping gene transcripts were measured during stationary phase in standard growth medium and during extreme nutrient starvation. These conditions differentially… CONTINUE READING

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