Assessment of real-time PCR for quantification of Legionella spp. in spa water.

@article{Guillemet2010AssessmentOR,
  title={Assessment of real-time PCR for quantification of Legionella spp. in spa water.},
  author={Thibault A Guillemet and Benoit L{\'e}vesque and Denis Gauvin and Nicholas Brousseau and J-P Giroux and Philippe Cantin},
  journal={Letters in applied microbiology},
  year={2010},
  volume={51 6},
  pages={639-44}
}
AIMS Legionella bacteria ubiquitously colonize natural freshwater and are responsible for legionellosis in humans. Several cases of legionellosis have been associated in particular with the use of whirlpool spas. The objective of this study was to verify whether real-time PCR is applicable for the quantification of Legionella spp. in spa water. METHODS AND RESULTS The study compared concentrations obtained by real-time PCR vs that obtained by conventional culture for 101 spa water samples… CONTINUE READING