Assessment of protein stability in cerebrospinal fluid using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry protein profiling

@article{Ranganathan2006AssessmentOP,
  title={Assessment of protein stability in cerebrospinal fluid using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry protein profiling},
  author={Srikanth Ranganathan and Anna Polshyna and Georgina C B Nicholl and James Lyons-Weiler and Robert Bowser},
  journal={Clinical Proteomics},
  year={2006},
  volume={2},
  pages={91-101}
}
Recent studies have evaluated proper acquisition and storage procedures for the use of serum or plasma for mass spectrometry (MS)-based proteomics. The present study examines the proteome stability of human cerebrospinal fluid (CSF) over time at 23°C (room temperature) and 4°C using surface-enhanced laser desorption/ionization time-of-flight MS. Data analysis revealed that statistically significant differences in protein profiles are apparent within 4 h at 23°C and between 6 and 8 h at 4°C… CONTINUE READING