Assessment of polysomaty, embryo formation and regeneration in liquid media for various species of diploid annual Medicago.

@article{Iantcheva2001AssessmentOP,
  title={Assessment of polysomaty, embryo formation and regeneration in liquid media for various species of diploid annual Medicago.},
  author={Anelia Iantcheva and Mariana Vlahova and Toan Hanh Trinh and Spencer Brown and Adrian Slater and Malcolm C. Elliott and Atanas Atanassov},
  journal={Plant science : an international journal of experimental plant biology},
  year={2001},
  volume={160 4},
  pages={621-627}
}
To avoid polyploidy in regenerants the source of explant material should be monosomatic. Therefore, the leaf and petiole tissue of five diploid Medicago species (Medicago ciliaris, Medicago murex, Medicago orbicularis, Medicago polymorpha and Medicago truncatula cv. Jemalong, and the ecotype R108-1) was assessed for polysomaty by flow cytometry. For the species studied the frequency of 2C nuclei was about 90% in leaves compared with that in petioles. Embryos were readily formed from tissue of… CONTINUE READING
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