Assessment of nuclear transfer techniques to prevent the transmission of heritable mitochondrial disorders without compromising embryonic development competence in mice.

@article{Neupane2014AssessmentON,
  title={Assessment of nuclear transfer techniques to prevent the transmission of heritable mitochondrial disorders without compromising embryonic development competence in mice.},
  author={Jitesh Neupane and Mado Vandewoestyne and Sabitri Ghimire and Yuechao Lu and Chen Qian and Rudy Noel Van Coster and Jan M R Gerris and Tom Deroo and Dieter Deforce and Petra de Sutter and Bj{\"o}rn Heindryckx},
  journal={Mitochondrion},
  year={2014},
  volume={18},
  pages={27-33}
}
To evaluate and compare mitochondrial DNA (mtDNA) carry-over and embryonic development potential between different nuclear transfer techniques we performed germinal vesicle nuclear transfer (GV NT), metaphase-II spindle-chromosome-complex (MII-SCC) transfer and pronuclear transfer (PNT) in mice. No detectable mtDNA carry-over was seen in most of the reconstructed oocytes and embryos. No significant differences were seen in mtDNA carry-over rate between GV NT (n=20), MII-SCC transfer (0.29 ± 0… CONTINUE READING