Assessment of nitric oxide production by measurement of [15N]citrulline enrichment in human plasma using high-performance liquid chromatography-mass spectrometry.

@article{Lagerwerf1998AssessmentON,
  title={Assessment of nitric oxide production by measurement of [15N]citrulline enrichment in human plasma using high-performance liquid chromatography-mass spectrometry.},
  author={Fija M Lagerwerf and Ron Wever and Herman J M van Rijn and Cornelis Versluis and Wigger Heerma and Johan Haverkamp and Hein A. Koomans and Ton J Rabelink and P. Boer},
  journal={Analytical biochemistry},
  year={1998},
  volume={257 1},
  pages={45-52}
}
Nitric oxide (NO) is formed by a class of NO synthases (NOS), which convert arginine into citrulline. A decreased in vivo NO availability can be the result of an increased NO inactivation or a decreased NO production. The latter can be assessed by measurement of isotopic enrichment of plasma citrulline during infusion of isotopically labeled arginine. The potential of high-performance liquid chromatography (HPLC) coupled to mass spectrometry (MS) to determine enrichments of [15N2]arginine and… CONTINUE READING
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