Assessment of new culture method for detection of Borrelia species from serum of lyme disease patients.

@article{Johnson2014AssessmentON,
  title={Assessment of new culture method for detection of Borrelia species from serum of lyme disease patients.},
  author={Barbara J. B. Johnson and Mark A. Pilgard and Theresa M. Russell},
  journal={Journal of clinical microbiology},
  year={2014},
  volume={52 3},
  pages={721-4}
}
A novel method of culturing spirochetes from the serum of U.S. Lyme disease patients was recently reported by Sapi and colleagues to have 94% sensitivity and 100% specificity for Borrelia species as assessed by microscopy and DNA sequence analysis of the pyrG gene (E. Sapi, N. Pabbati, A. Datar, E. M. Davies, A. Rattelle, and B. A. Kuo, Int. J. Med. Sci. 10:362-376, 2013). The majority of the spirochetes described were related by pyrG sequences to species of Borrelia previously undetected in… CONTINUE READING

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