Assessment of morphology, antigenicity, and nucleic acid integrity for diagnostic thyroid pathology using formalin substitute fixatives.

@article{Lassalle2009AssessmentOM,
  title={Assessment of morphology, antigenicity, and nucleic acid integrity for diagnostic thyroid pathology using formalin substitute fixatives.},
  author={Sandra Lassalle and V{\'e}ronique J Hofman and I. Marius and Virginie Gavric-Tanga and Patrick Brest and Katia Havet and Catherine Butori and Eric Selva and Jos{\'e} Santini and Baharia Mograbi and Paul Hofman},
  journal={Thyroid : official journal of the American Thyroid Association},
  year={2009},
  volume={19 11},
  pages={
          1239-48
        }
}
  • S. Lassalle, V. Hofman, P. Hofman
  • Published 4 November 2009
  • Medicine, Biology
  • Thyroid : official journal of the American Thyroid Association
BACKGROUND With the advent of the formaldehyde standard law in France, and because of the impact of new methods for diagnosis and prognosis in pathology, formalin replacement in surgical pathology laboratories is currently being discussed in France. However, a set of criteria must be assessed before introducing a formalin substitute fixative. The objective of this study was to compare formalin substitute fixation with formalin fixation and cryoconservation of tissues from several benign and… 

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References

SHOWING 1-10 OF 40 REFERENCES
Establishment of the formalin-free surgical pathology laboratory. Utility of an alcohol-based fixative.
TLDR
Alcohol-polyethylene glycol appears to be a satisfactory alternative to formalin in routine diagnostic surgical pathology and may be useful for diagnostic light microscopy.
Effects of Fixative and Fixation Protocols on Assessment of Her-2/neu Oncogene Amplification Status by Fluorescence In Situ Hybridization
TLDR
Non-formalin–based fixatives tested were associated with absent or technically compromised staining in at least one of the 3 neoplasms tested for each fixative when compared to the formalin-fixed control, and O-Fix did not seem to compromise staining quality in 3 paired specimens tested.
A Novel Fixative Improves Opportunities of Nucleic Acids and Proteomic Analysis in Human Archive's Tissues
TLDR
The results concerning the use of a new formalin free fixative, the FineFIX®, are presented, showing that the proteins obtained from FineFIX® treated samples are amenable and comparable in quality with those obtained from fresh frozen tissues.
Effect of formalin tissue fixation and processing on immunohistochemistry.
TLDR
If consistently applied, these procedures could eliminate some of the sources of variation in immunohistochemical stains and help to achieve standardization of fixation and processing.
RCL2, a new fixative, preserves morphology and nucleic acid integrity in paraffin-embedded breast carcinoma and microdissected breast tumor cells.
TLDR
Results indicate that methacarn, as previously shown, and RCL2, a promising new fixative, have great potential for performing both morphological and molecular analyses on the same fixed tissue sample, even after laser-capture microdissection, and can open new doors for investigating small target lesions such as premalignant breast lesions.
Effect of Formalin, Acetone, and RNAlater Fixatives on Tissue Preservation and Different Size Amplicons by Real-Time PCR from Paraffin-Embedded Tissue
  • C. PáskaK. Bögi Z. Schaff
  • Biology
    Diagnostic molecular pathology : the American journal of surgical pathology, part B
  • 2004
TLDR
The most adequate tissue preservation was found in case of formalin fixation, while there were no significant differences in the three fixatives’ yields for various size real-time PCR amplicons.
Immunohistochemistry of Tissue Prepared by a Molecular-Friendly Fixation and Processing System
TLDR
There is an enhancement of the sensitivity of few antibodies in UMFIX-exposed tissue, rendering antigen retrieval unnecessary, and increased sensitivity may be due to the effect of eliminating formalin from fixation and processing or the microwave energy.
Detection of proliferating cell nuclear antigen in paraffin-embedded specimens is dependent on preembedding tissue handling and fixation.
TLDR
It is concluded that the exceptional solubility of PCNA is responsible for reduced immunostaining in formalin-fixed material, that the loss is irreversible, and that methanol or methacarn is the fixative of choice for PCNA immunohistochemistry.
Impact of Fixative on Recovery of mRNA From Paraffin-Embedded Tissue
TLDR
Carnoy’s fixative is an excellent candidate for a new primary fixing reagent for human tissue samples and should replace formalin as the primary human tissue fixative, according to the data.
...
...