Assessment of membrane potential using confocal microspectrofluorimetry


Some slow potentiometric dyes, e.g., 3,3′-dipropylthiacarbocyanine and tetramethylrhodamine methyl ester, exhibit fluorescence spectral changes on redistributing from aqueous medium to cells. This effect has been used for spectroscopic discrimination of the emissions from free and bound dyes in cells. Such a discrimination can, in principle, allow for the… (More)
DOI: 10.1007/BF00862735


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@article{Plek1993AssessmentOM, title={Assessment of membrane potential using confocal microspectrofluorimetry}, author={Jarom{\'i}r Pl{\'a}{\vs}ek and Barbora Denksteinova and Franck Sureau}, journal={Journal of Fluorescence}, year={1993}, volume={3}, pages={157-159} }