Assessment of matrix effects and determination of niacin in human plasma using liquid-liquid extraction and liquid chromatography-tandem mass spectrometry.

@article{Peoples2008AssessmentOM,
  title={Assessment of matrix effects and determination of niacin in human plasma using liquid-liquid extraction and liquid chromatography-tandem mass spectrometry.},
  author={Michael C Peoples and Matthew S Halquist and Omnia A. Ismaiel and MAGDA Y. EL-MAMMLI and Abdalla A. Shalaby and H. Thomas Karnes},
  journal={Biomedical chromatography : BMC},
  year={2008},
  volume={22 11},
  pages={1272-8}
}
A simple, sensitive and rapid liquid-liquid extraction method for the analysis of nicotinic acid (niacin) and its labeled internal standard nicotinic acid-d4 (niacin-d4) in human plasma was developed and validated. The analyte and its internal standard were isolated from acidified plasma using a single liquid-liquid extraction procedure with methyl-t-butyl ether. The extracted samples were analyzed by liquid chromatography-tandem mass spectrometry in positive electrospray ionization mode with… CONTINUE READING