Assessment of hepatitis C virus quasispecies heterogeneity by gel shift analysis: correlation with response to interferon therapy.

@article{Polyak1997AssessmentOH,
  title={Assessment of hepatitis C virus quasispecies heterogeneity by gel shift analysis: correlation with response to interferon therapy.},
  author={Stephen J. Polyak and G Faulkner and Robert L. Carithers and Lawrence Corey and David R. Gretch},
  journal={The Journal of infectious diseases},
  year={1997},
  volume={175 5},
  pages={
          1101-7
        }
}
Hepatitis C virus (HCV) quasispecies heterogeneity was assessed in pretreatment sera of 22 patients treated with interferon (IFN). Quasispecies heterogeneity was quantitated by analysis of 490 hypervariable region 1 (HVR1) clones using gel shift analysis (GSA), which allowed determination of two components of HCV quasispecies heterogeneity: genetic complexity (number of variants) and genetic diversity (mean genetic distance between variants). HCV genotype and pretreatment RNA titer were similar… 
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HIV infection and antiretroviral therapy: effect on hepatitis C virus quasispecies variability.
TLDR
HIV coinfection is associated with decreased HCV quasispecies variability, which appears to be reversed by effective HAART, although HIV- and HAART-related effects on host immune pressure are likely to play a role in the observed differences in HCV genetic heterogeneity.
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TLDR
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